This paper presents the output voltage control and execution of a novel non-isolated high step-up (NIHS) DC-DC converter connected to a solar photovoltaic (PV) based DC microgrid system. The proposed converter provides a high output voltage conversion ratio over smaller duty cycles, small inductors, low cost, and high efficiency to enhance the level of the generated voltages of PV. Also, to overcome the drawback of PV, the detailed operation of maximum power point tracking (MPPT) for the novel boost DC-DC converter topology is presented. A control algorithm, modified perturb and observe (MP&O), is put forward to assure that the maximum power is extracted from PV at any environmental condition. It regulates the output voltage of the PV system to the desired DC bus voltage. This technique is compared with the Incremental Conductance (INC) and conventional P&O algorithm in terms of their computational complexity and oscillations near maximum power point (MPP) using MATLAB & Simulink. The focus is on the continuous conduction mode of the proposed converter. To demonstrate the effectiveness of the proposed converter, operation modes, and technical analysis are conducted. Also, the experimental results of a 200 W-12V/120V, 25 kHz prototype are given and discussed to justify the suggested converter.